Sperm Function Tests in the Stallion
نویسنده
چکیده
Introduction: There are many tests that describe semen quality in the stallion which include sperm motility, morphology, and longevity of motility and determination of total sperm numbers. In addition, recently, the flow cytometer (FC) has been used to evaluate various sperm compartment following staining with specific fluorescent probes that bind to different compartments of the sperm. Our lab is interested in developing sperm function tests that evaluate sperm compartments as well as determining how these tests can be applied clinically to the diagnosis of stallion subfertility. The sperm compartments that we are currently evaluating include the sperm DNA, the membrane integrity of the head and midpiece mitochondria, and the ability of the acrosome to react. The evaluation of these compartments allows the clinician additional diagnostic latitude when faced with a stallion that has a history of subfertility.
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